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Literature summary extracted from

  • Doly, J.; Le Roscouet, D.; Anagnostopoulos, C.
    Substrate specificity and adenosine triphosphatase activity of the ATP-dependent deoxyribonuclease of Bacillus subtilis (1981), Eur. J. Biochem., 114, 493-499.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.11.5 ATP 0.2 mM and higher concentrations inhibits phosphodiester hydrolysis; neither the rate nor the extent of hydrolysis of single-stranded DNA nor ATP is affected Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.11.5 0.15
-
 ATP
-
 Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.11.5 42500
-
 1 * 81000 + 1 * 70000 + 1 * 62000 + 1 * 52500 + 1 * 42500, no addition of protease inhibitor Bacillus subtilis
3.1.11.5 52500
-
 1 * 81000 + 1 * 70000 + 1 * 62000 + 1 * 52500 + 1 * 42500, no addition of protease inhibitor Bacillus subtilis
3.1.11.5 62000
-
 1 * 81000 + 1 * 70000 + 1 * 62000 + 1 * 52500 + 1 * 42500, no addition of protease inhibitor Bacillus subtilis
3.1.11.5 70000
-
 1 * 81000 + 1 * 70000 + 1 * 62000 + 1 * 52500 + 1 * 42500, no addition of protease inhibitor Bacillus subtilis
3.1.11.5 81000
-
 1 * 81000 + 1 * 70000 + 1 * 62000 + 1 * 52500 + 1 * 42500, no addition of protease inhibitor Bacillus subtilis
3.1.11.5 308000
-
 gel filtration, SDS-PAGE Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.11.5 ATP + H2O Bacillus subtilis
-
 ADP + phosphate
-
 ?
3.1.11.5 double-stranded DNA + H2O Bacillus subtilis
-
 single-stranded DNA fragments
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.11.5 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.11.5 to homogeneity Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.11.5 ATP + H2O
-
 Bacillus subtilis ADP + phosphate
-
 ?
3.1.11.5 double-stranded DNA + H2O
-
 Bacillus subtilis single-stranded DNA fragments
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.11.5 pentamer 1 * 81000 + 1 * 70000 + 1 * 62000 + 1 * 52500 + 1 * 42500, no addition of protease inhibitor Bacillus subtilis